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. 2007 Jun 15;21(12):1530–1545. doi: 10.1101/gad.1544207

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Copyright © 2007, Cold Spring Harbor Laboratory Press

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Figure 4. — H3K27me3 in parental macronucleus and macronuclear anlagen in conjugating Tetrahymena is RNAi dependent. Localization of H3K27me3 in anlagen (small white circles) at early anlagen formation (10 h conjugation) (A) and in parental macronuclei (large white circles) at micronucleus/macronucleus differentiation stages (6 h conjugation) (B). Wild-type, ΔEZL1, and RNAi-deficient (ΔTWI1 and ΔDCL1) mutants were processed for immunofluorescence staining with H3K27me3-specific antibody and counterstained with DAPI. Note the abnormal accumulation of H3K27me3 in the new micronuclei instead of macronuclear anlagen in ΔTWI1 and ΔDCL1 cells at both stages. (Mic) Micronuclei; (PM) parental macronuclei; (AN) macronuclear anlagen; (OM) old macronuclei.