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. 2007 Jun 27;2(6):e564. doi: 10.1371/journal.pone.0000564

Table 1. Bacterial Strains and Plasmids Used.

Bacterial Strains Relevant characteristics Reference
P. aeruginosa
PA01 Wild-type 46
PA01-gfp PA01 harboring pUCP19-gfp 17
flgE PA01 miniTn5Km2flgE, flagellar mutant This study
PA01 Wild-type M. Vasil [47]
PAO-fliC PAO1 fliC::Gmr 27
PAOC-fliC PAOC-complemented on the chromosome with the PA01 fliC gene at the attB site 49
PAO-fliD PAO1 fliD::Gmr 26
PA14 Wild-type 48
ΔmotAB motAB deletion strain of PA14 29
ΔmotCD motCD deletion strain of PA14 29
ΔmotAB ΔmotCD Both sets of mot genes, motAB and motCD deleted from PA14 29
flgK PA14 flgK::Tn5(Tcr) 32
CF 51 mucoid CF isolate This study
CF51N nonmucoid revertant of CF51 This study
CF90 mucoid CF isolate This study
CF90N nonmucoid revertant of CF90 This study
Plasmids
pUTminiTn5Km2 R6K-based suicide delivery plasmid, Kmr 19
pUCP19-gfp polylinker lacZ, laciq selection, bla, gfp 17
PVL1393 bla Invitrogen (Carlsbad, CA)