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(a) HPLC-chromatogram of RNA 4 synthesized with the TBDMS chemistry and the corresponding MS spectra of the separated fractions as indicated with an arrow. The reduced oligonucleotide strand shows a mass of 3947–3948 g mol−1 and the spin labeled strand of 3963 g mol−1. (b) HPLC-chromatogram and MS spectrum of RNA 4, synthesized with the ACE® chemistry without second HPLC purification. The calculated mass of the spin labeled RNA 4 is 3958.6 g mol−1.
