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: Agarose gel electrophoresis of PCR products (20 µl) obtained in the PCR sensitivity assay following two rounds of 40 cycles of amplification with M paratuberculosis IS900 primers P90+ and P91+. Extracted tisssue DNA from PCR negative OFG samples was spiked with serial 10-fold dilutions of M paratuberculosis DNA. Lanes 1-6, M paratuberculosis DNA at 100 pg (lane 1), 10 pg (lane 2), 1 pg (lane 3), 100 fg (lane 4), 10 fg (lane 5), 1 fg (lane 6); lane 7, 100 bp DNA ladder.
