Table 1.
Characterization of MEL cell transfectants
| Clone | Doxycycline | T(g)* | G1, %† | B+, %‡ | C, %§ |
|---|---|---|---|---|---|
| p15.25 | − | 12.2 | 31.6 | 2 | 1 |
| + | 12.4 | 31.7 | 1 | 2 | |
| p15.47 | − | 11.8 | 31.7 | 2 | 2 |
| + | 11.9 | 32.4 | 2 | 2 | |
| p16.8 | − | 11.9 | 33.4 | 1 | 2 |
| + | 24.3 | 50.6 | 3 | 2 | |
| p16.11 | − | 11.9 | 30.7 | 2 | 3 |
| + | 25.3 | 52.3 | 1 | 3 | |
| p21.12 | − | 11.8 | 29.6 | 2 | 2 |
| + | 55.5 | 33.3 | 34 | 36 | |
| p21.35 | − | 11.9 | 30.7 | 1 | 3 |
| + | 52.3 | 33.4 | 31 | 33 | |
| p27.16 | − | 12.1 | 28.3 | 2 | 2 |
| + | 42.1 | 84.9 | 1 | 3 | |
| p27.77 | − | 11.9 | 27.6 | 1 | 3 |
| + | 40.2 | 87.8 | 1 | 1 | |
| MELrtTA | − | 11.9 | 29.1 | 1 | 2 |
| + | 11.7 | 28.5 | 2 | 3 |
Doubling time [T(g)] was determined by counting cell numbers with a Coulter counter at intervals of 24 h in samples of the indicated transfectants incubated in the presence (+) or absence (−) of doxycycline for 14 days at 37°C.
Percentage of cells in (G1, %) was determined by fluorescence-activated cell sorter scan analysis as described in Materials and Methods on aliquots of cells incubated the presence (+) or absence (−) of doxycycline for 36 h at 37°C.
Percentage of benzidine-positive (B+) cells was determined as described in Materials and Methods on aliquots of cells incubated for 5 days.
Percentage of cells committed to terminal differentiation (C, %) was determined by plasma clot assays as described in Materials and Methods on aliquots of cells incubated for 4 days.