Table 2.

Effect of roscovitine and doxycycline treatment of p16 transfectants

Clone	Roscovitine*	Doxycycline*	B+, %†
p16.8	−	−	2
	−	+	2
	+	−	2
	+	+	84
p16.11	−	−	3
	−	+	1
	+	−	2
	+	+	85
MELrtTA	−	−	2
	−	+	3
	+	−	2
	+	+	2

^*The indicated cell types were first cultured in the presence (+) or absence (−) of 50 μM roscovitine for 12 h, followed by further incubation with or without roscovitine and the presence (+) or absence (−) of 1 μg/ml doxycycline, as indicated. 

^† Percentage of benzidine-positive (B+) cells was determined as described in Materials and Methods on aliquots of cells incubated for 5 days.