Table 2. ‘Insertion loop’ amino acid sequence, disulphide bridges and substrate binding pockets in CMN mycolyl-transferases.

Protein	‘Insertion loop’ amino acid sequence	Disulphide bridge	Trehalose 1151 binding residues							Trehalose 1152 binding residues
1F0P		Cys 87-	40D	43R	126S	223N	262H	263S	264W	154D	157Q	159M	231N	232F	235S	236S	239K
	Cys 92
Rv0129			38D	41R	124S	221N	260H	261S	262W	152N	155E	157W	229G	230L	233R	234T	237T
Rv3804		Cys 87-	40D	43R	126S	223N	262H	263S	264W	154D	157Q	159M	231G	232F	235T	236S	239K
		Cys 92
Ncgl2777	AIGPA		40D	43R	121S	216G	261H	262S	263W	149D	152S	154G	231V	232I	235M	236T	239T
Ce2709	ATGPA		40D	43R	121S	215G	261H	262A	263W	149D	152S	154G	231L	232I	235M	236T	239T
Ncgl2779	DH		41D	44R	128S	223V	266H	267G	268W	156N	159A	161G	236F	237V	240T	241S	244I
Ce2710	DH		41D	44R	128S	223T	266H	267S	268W	156T	159A	161G	236A	237V	240A	241T	244A
Ncgl0987	SEKEPFYN		41D	44G	125S	219D	267H	268N	269W	153S	156D	158I	240S	241C	244A	245L	248S
Ce1488	YADEPFYN		41D	44G	125S	219E	267H	268N	269W	153S	156D	158I	240S	241C	244A	245L	248A
Ncgl0885	DNAPIDEDAFKNR		41G	44D	124S	-	272H	273A	274W	152E	155S	157M	241A	242M	245T	246C	249N
Ce0984	ENAPEDEKGLKNR		41G	44D	124S	-	272H	273A	274W	152E	155S	157M	241A	242L	245T	246C	249N
Ncgl2101	DNAPIDEDAFKNR		41G	44D	124S	-	272H	273A	274W	152E	155S	157M	241A	242M	245T	246C	249N
Ncgl0336	SPRFEGLNQQVQSIAMAET		41N	44D	124S	218D	276H	277S	278W	152A	155S	157L	246A	247A	250K	251C	254D
Ce0356	SPRFNGLDQAYLSLAMTET		41N	44D	124S	218N	276H	277S	278W	152S	155Q	157L	246A	247A	250K	251C	254D
Nfa1810	FG		40D	43R	153S	249N	291H	292N	293W	181N	184A	186G	260V	261L	264A	265N	268A
Nfa1820	FN		40D	43R	148S	244S	286H	287A	288W	176N	179A	181G	255A	256L	259A	260N	263A
Nfa1830	SPVGVFN		39D	42R	124S	218N	264H	265S	266W	152N	155A	157G	234A	235L	238V	239N	242A
Nfa1840	PGVST		41D	44R	122S	217S	263H	264S	265W	150T	153T	155G	233I	234L	237L	238T	241N
Nfa25110		Cys 146-	38A	41G	120S	-	252H	253T	254W	148W	151D	153P	222A	223I	226T	227C	230A
		Cys 227
Nfa45560	APGIDGNPLDLVER	Cys 146-	38N	41D	120S	241T	266H	267S	268W	148R	151D	153A	237T	238V	241A	242C	245P
		Cys 242
Nfa7210	GPYALPGSYGLANQ	Cys 149-	41N	44G	123S	218N	271H	272S	273W	151Q	154D	156V	241A	242G	245Y	246C	249N
		Cys 246
Nfa38260	GPHAMPGSDGLTNQ	Cys 150-	41A	44G	123S	217N	270H	271S	272W	151Q	154D	156V	240A	241G	244H	245C	248N
		Cys 246
Nfa32420	YLNAAPGPMGAVN-	Cys 150-	41N	44D	123S	218Y	270H	271Y	272W	151Q	154D	156T	240A	241A	244Q	245C	248N
		Cys 246
Nfa23770	NPRLHNDDRQLLNQ	Cys 157-	41N	44G	130S	224A	278H	279S	280W	158M	161D	163L	247S	248V	241L	252C	255R
		Cys 253
Nfa43800	AVGGDPMQLGYQ	Cys 149-	41N	44S	122S	-	267H	268A	269W	150R	153D	155Q	237A	238V	241M	242C	245Q
		Cys 243
Nfa30260	GPGIDADPLALADQ	Cys 149-	41N	44T	123S	217Q	270H	271S	272W	151P	154D	156R	240A	241V	244D	245C	248E
		Cys 245
Nfa5610	KPQLAEN	Cys 148-	41D	43D	122S	214L	260H	261S	262W	150D	153L	155T	230V	231G	234I	235C	238A
		Cys 235
Dip0365	SPRLAGKDPVTIFATNLIT		39N	42D	122S	216S	274H	275S	276W	150A	153S	155L	244A	245G	248M	249C	252D
Dip2339	PKEDGPFT		41D	44T	125S	219G	269H	270S	271W	153S	156N	158S	240R	241C	244E	245L	248S
Dip2193	ANKKG		40D	43R	121S	215G	261H	262D	263W	149D	152S	154G	231V	232I	235M	236T	239T
Dip2194	ND		41D	44R	125S	220Y	263H	264N	265W	153S	156V	158G	233I	234A	237V	238S	241I