Figure 1.

E1A is acetylated by p300, P/CAF, and Gcn5. (a) Recombinant E1A was incubated with full-length mouse p300 expressed in baculovirus-infected SF9 cells or human P/CAF catalytic domain expressed in bacteria in the presence of ³H-AcCoA for 30 min at 30°C. The reaction was subjected to SDS/PAGE analysis and fluorography. Autoacetylation of p300 was shown at the top of the gel. (b) Comparison of E1A and histone acetylation by different HATs. Recombinant E1A or core histones purified from chicken blood were incubated with full-length mouse p300, yeast Gcn5 catalytic domain, or P/CAF catalytic domain in the presence of ³H-AcCoA for 30 min at 30°C. The reaction was subjected to SDS/PAGE analysis and fluorography.