Table 2. Prediction accuracies of the Gram- individual location predictors for the negative test sets. The results of highest accuracy are shown in bold.

Sequence representation	Sub-sequence length	Cytoplasmic accuracy (%)	Inner Membrane accuracy (%)	Periplasmic accuracy (%)	Outer Membrane accuracy (%)	Extra-celluar accuracy (%)
Amino acid composition	10	51.03	83.52	44.02	37.85	73.31
	20	53.64	81.09	58.23	51.67	76.90
	30	64.07	84.24	62.68	64.69	85.02
	40	81.75	88.31	79.43	77.11	86.42
	50	90.13	94.76	92.01	86.36	92.85
	All sequence	88.24	93.41	84.42	86.02	88.22
Actual amino acids	10	40.03	53.59	20.52	12.05	23.24
	20	41.49	58.21	23.84	16.73	22.86
	30	48.79	68.84	55.08	22.41	26.72
	40	55.32	61.33	42.21	25.62	30.55
	50	58.62	63.71	49.33	32.41	29.86
	All sequence	64.28	59.35	43.57	34.79	30.08
Normalised amino acid composition	10	44.63	84.04	41.93	44.32	74.59
	20	48.32	88.68	56.26	46.72	77.35
	30	61.04	87.14	63.17	51.48	81.68
	40	71.38	93.73	71.87	56.37	84.24
	50	77.20	96.26	78.88	68.53	85.32
	All sequence	83.56	92.47	73.29	59.25	84.99