Maternal inheritance of eve stripe boundaries. Genetic analysis of D. melanogaster Fra and Ind reciprocal crosses to F3s. (A) Inheritance of absolute stripe position is consistent with an additive, maternally inherited trait. Line means for absolute measures of stripe positions (μm from anterior pole), shown as the deviation from the grand mean, for each stripe. The designation f (Fra) or i (Ind) after a cross (F1f, F1i, etc.) indicates the maternal lineage. The stripe locations in F1f and F1i lines resemble those of their mothers, Fra and Ind; stripes in F2 and F3 lines are localized midway between the parental lines. (B) F3 embryos exhibit a wide range of embryo lengths (as expected for a segregating trait) but retain nearly perfect linear scaling of eve stripe boundary locations across this length distribution. Relative stripe positions in individual F3 embryos for the anterior boundaries of each eve stripe are plotted against each embryo's length.