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. 2001 Apr;158(4):1391–1398. doi: 10.1016/S0002-9440(10)64090-4

Figure 1.

Figure 1.

Demonstration of the Vα24+ T cell clonalities and the invariant Vα24JαQ TCR in peripheral blood of controls (A), peripheral blood of periodontitis patients (B), gingival tissue of controls (C), and gingival tissue of periodontitis patients (D). Total RNA was extracted from PBMCs and gingival tissues and then subjected for RT-PCR-SSCP analysis. The top and bottom panels demonstrate the total Vα24+ TCR SSCP profiles as detected with a Cα probe and the invariant Vα24JαQ band as detected with the JαQ-specific probe, respectively. The cloned Vα24JαQ DNA fragment as a positive control was applied on the far left lanes. The alphabetical code and the numerical code correspond to each control (A and C) and each patient (B and D), respectively, implying that blood samples and gingival tissues were taken from same subjects and patients. Arrows indicate the position for the invariant Vα24+JαQ TCR. The unique bands were indicated by arrowhead. Another unique band appeared in patients 8 and 14 was indicated by open arrowhead.