Figure 9.

Colocalization of C1q with fibrillar Aβ plaques and associated microglia in 12- (A and D) or 7-month-old (C and F) PS/APP mouse brain. C1q-immunostained sections were counterstained with thioflavin-S to detect fibrillar Aβ. To examine the cellular location of C1q, one section was incubated sequentially with fluorescently labeled C1q (C) and F4/80 (F) which was then detected with DAB. C1q appears to colocalize both with plaques and with microglia. Scale bars, 50 μm (in A for A, B, D, and E) and 25 μm (in C for C and F).