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. 2001 Apr;158(4):1263–1270. doi: 10.1016/S0002-9440(10)64077-1

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Copyright © 2001, American Society for Investigative Pathology

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Figure 7. — Immunoelectron microscopy. Results shown in A to D are representative figures of five cases. A: CCR4⁺ lymphocytes (arrowheads), labeled by immunoperoxidase method as linear black deposits, are located around dendritic-shaped cells (DC) in the inflamed dermis. L, all lymphocytes in this figure. B: CD1a⁺ DC is labeled by small, black spherical particles on the plasma membrane by silver-enhanced gold particle method. C and D: Double-labeling immunoelectron microscopy for MDC and CD1a. MDC is expressed in the perinuclear space (arrowheads) and the rough endoplasmic reticulum (arrows) by diffuse black deposits by immunoperoxidase method, whereas CD1a is expressed along the plasma membrane by dot-like silver-enhanced gold particles (enlarged in D), identifying this cell as DC. Original magnifications, ×3,000 (A), × 2,000 (B), ×5,000 (C), ×12,000 (D). Scale bars, 1 μm.