Table 1.
Expression of Matriptase and HAI-1 in Breast Cancer Cell Lines and Comparison with Markers of Differentiation and in Vitro Invasiveness
| Cell line | ER | Matriptase | HAI-1 | E-cadherin | ZO-1 | Vimentin | In vitro invasion* |
|---|---|---|---|---|---|---|---|
| A1N4 | − | + | + | NA | NA | NA | NA |
| MCF-10A | − | + | + | NA | NA | NA | NA |
| MCF-7 | + | + | + | + | + | − | + |
| T47D | + | + | + | + | + | − | + |
| ZR-75-1 | + | + | + | + | − | − | + |
| BT-474 | + | + | + | + | − | − | + |
| SKBR3 | − | + | + | − | + | − | + |
| MDA-MB-468 | − | + | + | − | − | − | + |
| MDA-MB-453 | − | + | + | − | − | − | + |
| MDA-MB-157 | − | − | − | − | NA | NA | NA |
| MDA-MB-436 | − | − | − | − | + | + | ++ |
| MDA-MB-435 | − | − | − | − | − | + | ++ |
| MDA-MB-231 | − | − | − | − | − | + | +++ |
| BT-549 | − | − | − | − | − | + | +++ |
| Hs578t | − | − | − | − | − | + | +++ |
The expression of matriptase and HAI-1 in immortalized human breast epithelial cell lines and cancer cell lines as determined in Figure 1 ▶ are compared with the expression of E-cadherin and zona occludens-1 (ZO-1)—markers typical of an epithelial differentiation, and vimentin—a marker typical of mesonchymal differentiation, as determined by others. 34-36
Matriptase and HAI-1 are expressed in the same cells that express either E-cadherin or ZO-1, or which lack expression of vimentin.
Abbreviations: ER, estrogen receptor status; NA, data not available.
*In vitro invasiveness as assessed in vitro by invasion into the extracellular matrix preparation Matrigel, as determined by Sommers et al. 34