Table 1.

Ectopic expression of cav-1 reduced iNOS activity in HT29 cells

Cell line	Activity in vitro	Activity in situ
HT29	33.6  ± 13.8	8.2  ± 1.2
Mock	25.2  ± 7.5	7.6  ± 1.4
C13	6.5  ± 1.5*	4.5  ± 0.8*
C16	5.4  ± 3.3*	4.5  ± 1.4*

iNOS activity was assessed in vitro by measuring [³H]arginine conversion to [³H]citrulline (picomoles citrulline per minute per milligram of protein) or in situ by measuring nitrite accumulation in the conditioned medium (nanomoles nitrite per milligram of protein) of parental cells (HT29), mock-transfected cells, or cav-1-transfected (C13 and C16) cells stimulated during 15 h by IL-1β, IL-6, and IFNγ. Mean ± SEM; n = 3–5; *, P < 0.05, t test vs. HT29 or mock.