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. 2001 Apr;158(4):1379–1390. doi: 10.1016/S0002-9440(10)64089-8

Figure 3.

Figure 3.

A: The effect of cyclosporine and tacrolimus on the growth of human BECs was tested on human bile-duct tree explants (hBDT), as described in the Materials and Methods. On average, five explant cultures were included in each treatment group. Measurements of culture size were made every 2 days for 6 days. Note the increase in culture size in both the control and tacrolimus-containing media, compared to the significant decrease in size of the cyclosporine-treated cultures (*, P < 0.02; **, P < 0.006 compared to control or tacrolimus-treated cultures). B: To show that the diminished size of the BEC cultures was related to mito-inhibition, the cultures were also labeled with BrdU for either 6 or 12 hours before fixation for anti-BrdU staining. Note the significantly lower percentage of BrdU+ BEC nuclei in the cyclosporine-treated cultures, compared to the others (*, P < 0.01 versus BEC cultures treated with 1,000 ng/ml cyclosporine).