Figure 7.

Effect of in vivo IFN-γ depletion on MIG and IP-10 transcript levels in lungs with day 2 Ag-bead granulomas. Five individual mice are presented in each type. As indicated, three of the mice are treated with anti-IFN-γ and two with control IgG. The expression levels were determined by PCR-ELISA analysis and the results are presented in arbitrary units (AU) as described in Materials and Methods. Because of the low expression levels in the type-2 lungs, a higher degree of amplification (30 cycles for type 2 versus 28 cycles for type 1) was used for detection and revealed that some effect was achieved with IFN-γ neutralization.