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. 2001 Apr;158(4):1217–1222. doi: 10.1016/S0002-9440(10)64071-0

Figure 2.

Figure 2.

a: RT-PCR of GHR expression in six breast cancer cases. Lanes 2, 4, 6, 8, 10, and 12 represent RT-PCR of tumor tissues. Lanes 3, 5, 7, 9, 11, and 13 represent no template controls. Lane 1 shows a DNA marker (1 kb, GIBCO BRL). The full-length GHR renders a fragment of 201 bp (lanes 2, 4, 6, and 8). The GHR without exon 3 renders a fragment of 135 bp (lane 10). Lane 12 represents a case in which both forms are expressed. b: Digestion of the PCR fragment with Bsp 1286 I. Cleavage occurs in the third exon, resulting in two fragments that are 119 and 82 bp in size (lanes 2–5) or, in the case of exon 3 deletion, leaving the fragment undigested (lane 6). Lane 1 is the DNA marker X174 HaeIII.