Table 1.

Sequences of the Oligonucleotide Primers Used in this Study

NF2 gene	Primer	Sequence	Annealing temperature [°C]
Exon 1	NF2.1F1	5′ GCT-AAA-GGG-CTC-AGA-GTG-CA 3′	60
	NF2.1R2	5′ AGC-TTC-CAC-CTC-GAC-TGT-CA 3′
Exon 2	NF2.2F1	5′ AGA-GTG-GAG-AGT-GCA-GAG-AA 3′	50
	NF2.2R2	5′ CTC-TAC-TAT-ACA-GCT-ACA-GCG-C 3′
Exon 3	NF2.3F1	5′ TAG-CAC-AGG-AGG-AAG-TGC-CAA-T 3′	50
	NF2.3R1	5′ CAA-GTT-CTC-TCA-GAA-CTG-GG 3′
Exon 4	NF2.4F1	5′ ATG-TCT-CCC-TTG-TTG-CTC-CT 3′	57
	NF2.4R1	5′ GAG-TGA-TCC-CAT-GAC-CCA-AA 3′
Exon 5	NF2.5F1	5′ AAT-CTC-AAT-CGC-CTG-CTC-TC 3′	55
	NF2.5R1	5′ CCA-CAT-ATC-TGC-TAT-GTC-TTC-CTG 3′
Exon 6	NF2.6F1	5′ TGA-CTA-TCT-CCC-TGG-GTG-TA 3′	50
	NF2.6R1	5′ AAA-CCA-ACA-ATG-AAT-GGG-CC 3′
Exon 7	NF2.7F1	5′ TCC-AAT-GAC-AGT-GTC-TTC-CG 3′	50
	NF2.7R1	5′ TAC-ACA-AGG-AGC-TCA-GAG-AG 3′
Exon 8	NF2.8F1	5′ TGT-TGG-GAC-CTG-CTG-AAA-CT 3′	50
	NF2.8R1	5′ CCC-ATC-TGC-AGT-ACA-CAC-AT 3′
Exon 9*	NF2.9F3	5′ GTT-CTG-CTT-CAT-TCT-TCC 3′	45
	NF2.9R2	5′ GTA-ATG-AAA-ACC-AGG-ATC 3′
Exon 10*	NF2.10F2	5′ CCT-TTT-TGT-CTG-CTT-CTG 3′	45
	NF2.10R2	5′ TCA-GTT-AAA-ACA-AGG-TTG 3′
Exon 11	NF2.11F1	5′ TCT-CGA-GCC-CTG-TGA-TTC-AA 3′	50
	NF2.11R2	5′ AAG-TCC-CCA-AGT-AGC-CTC-CTG-GAA 3′
Exon 12	NF2.12F1	5′ CTT-CAG-CTA-AGA-GCA-CTG-TG 3′	50
	NF2.12R3	5′ AGG-ACA-ACT-GCT-GTA-GAG-CT 3′
Exon 13	NF2.13F1	5′ GCT-GAC-ATC-TCA-TCC-TTT-CC 3′	55
	NF2.13R1	5′ AAC-ATC-ACC-AGG-ACT-AAG-GC 3′
Exon 14	NF2.14F1	5′ GTC-CTT-CTG-TGC-TTG-TAT-GA 3′	55
	NF2.14R1	5′ GTT-CAC-AGC-TGC-CCA-CAG-CA 3′
Exon 15	NF2.15F1	5′ TGA-GCC-GTG-TCT-CAC-TGT-CT 3′	55
	NF2.15R1	5′ TAA-TGA-TGG-TCC-TGA-TCA-GC 3′

F, forward; R, reverse.

*Primer sequences previously published.¹⁵