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. 2001 Apr;158(4):1271–1278. doi: 10.1016/S0002-9440(10)64078-3

Figure 4.

Figure 4.

Fluorescent micrographs of AIF immunohistochemistry. The eyes were stained by AIF antiserum and visualized by fluorescent microscopy (see Materials and Methods). A: Control retina stained by AIF-antiserum. B: Detached retina on day 3 stained by AIF-antiserum. C: Control for staining by pre-immune serum. D: Control by pre-absorbed antiserum (with 1 μg/μl recombinant AIF). E: Double staining for AIF (green) and GFAP (red) on day 1 after RD. Specific AIF-staining is shown in ganglion cells, inner nuclear layer, outer plexiform layer, and photoreceptors (A, arrows). In normal photoreceptors, AIF staining is localized in inner segment, showing a multilinear pattern (A, arrowheads). Note the AIF-positive photoreceptor nuclei in the outer nuclear layer of detached retina (B, arrows). AIF staining decreased from the inner segment and appeared in the nucleus. Original magnifications: ×400 (A, B, and E), ×200 (C and D).