Table 1.

Sequences of Primers Used in This Study

VH con	5′-CTGTCGACACGGCCGTGTATTACTG-3′
JH con	5′-AACTGCTGAGGAGACGGTGACC-3′
Vγ 1–8	5′-ACCAGGAGGGGAAGGCCCCACAG-3′
Vγ 9	5′-GGAAAGGAATCTGGCATTCCG-3′
Vγ 10	5′-AATCCGCAGCTCGACGCAGCA-3′
Vγ 11	5′-GCTCAAGATTGCTCAGGTGGG-3′
Jγ 1/2	5′-ACCTGTGACACCAAGTGTTGTTC-3′
Jγ P1/2	5′-AGTTACTATGAGCT(CT)TAGTCCC-3′
Jγ P	5′-TGTAATGATAAGCTTTGTTCC-3′
Vβ 1/5	5′-ACAGCAAGTGAC〈TAG〉CTGAGATGCTC-3′
Vβ 2	5′-GAGTGCCGTTCCCTGGACTTTCAG-3′
Vβ 3	5′-GTAACCCAGAGCTCGAGATATCTA-3′
Vβ 4	5′-TCCAGTGTCAAGTCGATAGCCAAGTC-3′
Vβ 6.a	5′-ATGTAACT〈CT〉TCAGGTGTGATCCAA-3′
Vβ 6.b	5′-GTGTGATCCAATTTCAGGTCATAC-3′
Vβ 7	5′-TACGCAGACACCAA〈GA〉ACACCTGGTCA-3′
Vβ 8	5′-GGTGACAGAGATGGGACAAGAAGT-3′
Vβ 9	5′-CCCAGACTCCAAAATACCTGGTCA-3′
Vβ 10	5′-AAGGTCACCCAGAGACCTAGACTT-3′
Vβ 11	5′-GATCACTCTGGAATGTTCTCAAACC-3′
Vβ 12	5′-CCAAGACACAAGGTCACAGAGACA-3′
Vβ 13	5′-GTGTCACTCAGACCCCAAAATTCC-3′
Vβ 14	5′-GTGACCCAGAACCCAAGATACCTC-3′
Vβ 15	5′-GTTACCCAGACCCCAAGGAATAGG-3′
Vβ 16	5′-ATAGAAGCTGGAGTTACTCAGTTC-3′
Vβ 17	5′-CACTCAGTCCCCAAAGTACCTGTT-3′
Vβ 18	5′-TGCAGAACCCAAGACACCTGGTCA-3′
Vβ 19	5′-ACAAAGATGGATTGTACCCCCGAA-3′
Vβ 20	5′-GTCAGATCTCAGACTATTCATCAATGG-3′
Vβ 21	5′-CAGTCTCCCAGATATAAGATTA〈TC〉AGAG-3′
Vβ 22	5′-GGTCACACAGATGGGACAGGAAGT-3′
Vβ 23	5′-CTGATCAAAGAAAAGAGGGAAACAGCC-3′
Vβ 24	5′-CAAGATACCAGGTTACCCAGTTTG-3′
Vβ 25	5′-GACAGAAAGCAAAATTATATTGTGCC-3′
3′Jβ 1.2	5′-TACAACGGTTAACCTGGTCCCCGA-3′
5′Jβ 1.2	5′-TAACCTGGTCCCCGAACCGAAGG-3′
3′Jβ 1.3	5′-CACCTACAACAGTGAGCCAACTT-3′
5′Jβ 1.3	5′-GCCAACTTCCCTCTCCAAAATATATGG-3′
3′Jβ 1.5	5′-CCAACTTACCTAGGATGGAGAGTCGA-3′
5′Jβ 1.5	5′-GATGGAGAGTCGAGTCCCATCAC-3′
3′Jβ 1.6	5′-CCTGGTCCCATTCCCAAAGTGGA-3′
5′Jβ 1.6	5′-CCCATTCCCAAAGTGGAGGGGTG-3′
3′Jβ 2.2	5′-CCTTACCCAGTACGGTCAGCCTA-3′
5′Jβ 2.2	5′-AGTACGGTCAGCCTAGAGCCTTCT-3′
3′Jβ 2.6	5′-CAGCCGCCGCCTTCCACCTGAAT-3′
5′Jβ 2.6	5′-CGGCCCCGAAAGTCAGGACGTT-3′
3′Jβ 2.7	5′-TCCATCGTTCACCTTCTCTCTAAACA-3′
5′Jβ 2.7	5′-GCCCGAATCTCACCTGTGACCGTG-3′
Jβ 2.4	5′-CCAGCTTACCCAGCACTGAGAGC-3′

〈 〉 denotes a mixture of nucleotides at that position.

Vβ primers 7, 9, and 18 expectedly cross-react with members of all of these three Vβ families and are, therefore, used in a mix at a concentration of 14 nmol/L and 50 nmol/L each for first round and second round amplifications, respectively.