Table 3.
Single-Cell PCR Analysis in AILD
| Case | Single CD4+ cells | Single CD8+ cells | Single Ki67+ cells | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Number of specific PCR products/ number of cells | Functional/ nonfunctional sequences | Clonal*/unique sequences | Number of specific PCR products/ number of cells | Functional/ nonfunctional sequences | Clonal*/unique sequences | Number of specific PCR products/ number of cells | Functional/ nonfunctional sequences | Clonal*/unique sequences | |
| 1 | 9 /32 | 9 /0 | 7 /2 | 12 /32 | 10 /2 | 0 /10 | 3 /32 | 3 /0 | 3 /0 |
| 2 | 2 /32 | 2 /0 | 2 /0 | 9 /52 | 6 /3 | 0 /7 | 3 /48 | 3 /0 | 3 /0 |
| 3 | 11 /32 | 9 /2 | 6 /3 | 19 /84 | 13 /6 | 2 /11 | 6 /32 | 6 /0 | 5 /1 |
| 4 | 16 /48 | 15 /1 | 0 /15 | 12 /32 | 8 /4 | 0 /8 | 19 /48 | 17 /2 | 0 /17 |
| 5 | 8 /48 | 8 /0 | 5 /3 | 7 /48 | 5 /1† | 0 /5 | 11 /44 | 11 /0 | 7 /4 |
| 6 | 23 /72 | 20 /3 | 0 /20 | 10 /72 | 8 /1† | 0 /8 | 4 /72 | 4 /0 | 0 /4 |
| 7 | 11 /54 | 11 /0 | 0 /11 | 13 /36 | 12 /† | 0 /12 | 5 /54 | 3 /† | 0 /3 |
| Controls‡ | 0 /106 | 0 /118 | 0 /110 | ||||||
*In each of the four cases harboring clones, the same clonal rearrangements were detected in the CD4+ and Ki67+ cells. Only potentially functional sequences were considered.
†The reading frame of a few sequences could not be unequivocally determined.
‡Controls include aliquots of the buffer covering the sections during micromanipulation and PCR buffer.