Figure 2.
Western blots of cytoplasmic extracts of yeast co-expressing fusion proteins that consisted of the pLexA DNA-binding domain and a fragment of human THP and the LexA activation domain fused to κ light chains. Top: The yeast expressed the κ light chain/LexA activation domain proteins (ITPBL5, ITPBL11, BC Syn 9, and SSH23). Bottom: Expression of the THP/pLexA DNA-binding domain fusion protein. The expected contribution of the κ light chain and THP fragments to each of the fusion proteins was ∼23 kd and 29 kd, respectively.