Table 2.
Primers Used to Generate the λ Light Chain Constructs
| Light chain construct, λ | Primer name | Forward and reverse primers |
|---|---|---|
| ITPBLL1 | LB75 | 5′-CCGGAATTCTCCGAATTCTCCTCTCTCACTGCACAG-3′ |
| LB69 | 5′-ACGCTCGAGGGGAATTCGCTCCCGGGTAGAAGTCTCT-3′ | |
| ITPBLL1a | LB75 | 5′-CCGGAATTCTCCGAATTCTCCTCTCTCACTGCACAG-3′ |
| PBLL1a | 5′-ACGCTCGAGTCGCTCAGGGATCTCTGA-3′ | |
| ITPBLL1b | PBLL1b | 5′-CCGGAATTCAGCGACCGGCCCTCAGAG-3′ |
| LB69 | 5′-ACGCTCGAGGGGAATTCGCTCCCGGGTAGAAGTCTCT-3′ | |
| ITPBLL1c | PBLL1b | 5′-CCGGAATTCAGCGACCGGCCCTCAGAG-3′ |
| PBLL1c | 5′-ACGCTCGAGTAGGACGGTGACCTT-3′ | |
| ITPBLL1d | LB75 | 5′-CCGGAATTCTCCGAATTCTCCTCTCTCACTGCACAG-3′ |
| PBLL1d | 5′-ACGCTCGAGATAGACGACCAGCAC-3′ | |
| LKPBLLL2 | LB75 | 5′-CCGGAATTCTCCGAATTCTCCTCTCTCACTGCACAG-3′ |
| LB69 | 5′-ACGCTCGAGGGGAATTCGCTCCCGGGTAGAAGTCTCT-3′ | |
| ITPBLL11 | LB76 | 5′-CCGGAATTCTCCGAATTCTCTGCACAGTCTCTGAGGCC-3′ |
| LB69 | 5′-ACGCTCGAGGGGAATTCGCTCCCGGGTAGAAGTCTCT-3′ | |
| ITPBLL22 | LB77 | 5′-CCGGAATTCCCCTGAATTCCTCGGCGTCCTTGCTTACTGCA-3′ |
| LB69 | 5′-ACGCTCGAGGGGAATTCGCTCCCGGGTAGAAGTCTCT-3′ | |
| ITPBLL68 | LB75 | 5′-CCGGAATTCTCCGAATTCTCCTCTCTCACTGCACAG-3′ |
| LB69 | 5′-ACGCTCGAGGGGAATTCGCTCCCGGGTAGAAGTCTCT-3′ | |
| ITPBLL7 | LB75 | 5′-CCGGAATTCTCCGAATTCTCCTCTCTCACTGCACAG-3′ |
| LB69 | 5′-ACGCTCGAGGGGAATTCGCTCCCGGGTAGAAGTCTCT-3′ | |
| ITPBLL75 | LB75 | 5′-CCGGAATTCTCCGAATTCTCCTCTCTCACTGCACAG-3′ |
| LB69 | 5′-ACGCTCGAGGGGAATTCGCTCCCGGGTAGAAGTCTCT-3′ |
Underlined sequences contained Eco RI and Xho I restriction sites on the forward and reverse primers, respectively.