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. 2001 May;158(5):1665–1675. doi: 10.1016/S0002-9440(10)64122-3

Figure 5.

Figure 5.

In situ hybridization of rab38 in rat lung tissue. Perfused and excised rat lungs were fixed with 4% paraformaldehyde, embedded in paraffin, and cut into 5- to 7-μm-thick slices. The sections were hybridized with digoxigenin-labeled cRNA probes and then treated with RNase. The sections were treated with sheep anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. Color development was performed in 5-bromo-4-chloro-3-indolyl phosphate and nitroblue tetrazolium salt. A: Antisense RNA probe (original magnification, ×40). Some of the positive airway cells are indicated by arrowheads. B: Sense RNA probe (original magnification, ×40). Insets are original magnification of ×200 of the boxed areas. C: Antisense RNA probe (original magnification, ×200). Positive alveolar corner cells are indicated by arrows. D: Sense RNA probe (original magnification, ×200).