Figure 7.

Role of Bax and Bcl-2 in apoptosis in mutant osteoblasts. The levels of the pro-apoptotic protein Bax and the anti-apoptotic protein Bcl-2 were determined by Western blot analysis in Apert (Ap) and control (Co) cells in basal culture conditions, the bands were scanned and the ratio of Bax/Bcl-2 was determined (A). Bax protein levels were determined in Ap cells in the presence of specific neutralizing IL-1α and IL-1β antibodies (15 μg/ml each) or IgG, and the levels were corrected for β-actin (B).