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. 2001 Jun;158(6):1929–1935. doi: 10.1016/S0002-9440(10)64661-5

Figure 1.

Figure 1.

WB-F344 cells engraft in the myocardium. Engrafted WB-F344 cells express β-galactosidase (a–c) and range from small undifferentiated cells to long striated cells that measure up to 110 μm in length (b). β-galactosidase-expressing myocytes before dissection by LCM (c, asterisks) and after (d, asterisks) were used as a template to amplify by PCR a rat Y-chromosome-specific repetitive DNA sequence. e: Agarose electrophoresis of the PCR product. Unlike myocytes from control female mice (lane 5) but like WB-F344 cells grown in culture (lane 2), β-galactosidase-expressing myocytes (lanes 1 and 3) contain multimeters of the rat Y-chromosome repetitive 200-bp sequence (200, 400, 600 bp). e: lane 1, PCR product from four blue WB-F344-derived myocytes dissected by LCM from a female mouse donor heart section; lane 2, PCR product from WB-F344 cells in culture; lane 3, PCR product from two blue WB-F344-derived myocytes dissected by LCM from a female mouse donor heart section; lane 4, molecular weight standard (New England Biolabs, Beverly, MA); lane 5, PCR product from 20 random control native myocytes. f: A β-galactosidase-expressing myocyte demonstrates, after permeabilization and incubation with mAb 13-11, fluorescent sarcomeric striations (g, arrow) in the same cell. Scale bar, 25 μm.