Figure 2.

DCoH/PCD-protein is expressed in the melanoma cell line BLM34. a: Detection of DCoH/PCD in a NIH3T3 cell after transfection with a DCoH/PCD expression vector and in a BLM34⁴⁵ cell (b) using rabbit polyclonal antibodies raised against DCoH/PCD and a secondary Cy3-labeled antibody. Top: Co-staining of DCoH/PCD and DNA (Hoechst 33342). Middle and bottom: The separate signals for DCoH/PCD and DNA, respectively. DCoH/PCD staining was observed in the nucleus and the cytoplasma. c: Western blot using DCoH/PCD-specific antibodies as the primary antibody before (lanes 1 and 2) and after (lanes 3 and 4) preincubation with recombinant DCoH/PCD protein to detect DCoH/PCD in whole-cell extracts of BLM34 cells and Xenopus liver.