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FRAP is a cytoplasmic–nuclear shuttling protein. (A) CV-1 cells transiently transfected with FLAG-FRAP cDNA without (a) or with (b) treatment with 10 ng/ml LMB for 12 h were analyzed by immunocytochemistry and subsequent confocal microscopy. (B) Endogenous FRAP was analyzed on Western blotting on subcellular fractionation of both CV-1 and HEK293 cells. Anti-tubulin blot of the same fractions from CV-1 cells served as a control for the fractionation. C, cytoplasmic; N, nuclear.
