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. 2000 Dec 12;97(26):14340–14345. doi: 10.1073/pnas.011511898

Figure 3.

Figure 3

Subcellular localization and kinase activity of FRAP proteins tagged with various translocation signals. (A) Myc-tagged NLS-, NLS′-, NES-, and NES′-FRAP proteins were transiently expressed in CV-1 cells, followed by immunostaining and confocal microscopic analysis. (B) The same recombinant FRAP proteins were transiently expressed in HEK293 cells and immunoprecipitated by an anti-Myc antibody, and in vitro autokinase assays were performed in the immune complexes. All FRAP constructs, including wild-type, contained the S2035T mutation.