Figure 10.

Effect of inhibiting tyrosine phosphatases with pervanadate on dephosphorylation during hypoxia. Tubules were subjected to 60 minutes of hypoxia (H) with NES without or with 120 μmol/L of pervanadate (PERV). Samples collected at the end of hypoxia were immunoblotted for pY (A) and paxillin (B) and immunostained for pY (C). TC indicates oxygenated time control samples corresponding to the start of hypoxia at 15 minutes of incubation and the end of hypoxia at 75 minutes of incubation. Each lane in the immunoblot is from a separate flask subjected to the indicated conditions.