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Immunoblot for total β1 integrin. Tubules were incubated under oxygenated control conditions for 75 minutes (75′ TC), for 60 minutes of hypoxia, or 60 minutes of hypoxia and 60 minutes reoxygenation with NES during either during hypoxia or reoxygenation. H, sample taken at the end of 60 minutes hypoxia; R, sample taken after 60 minutes hypoxia followed by 60 minutes reoxygenation. ATP was added to the medium during reoxygenation.
