Table 1.
Percentage of CpG methylation in the LEP, PPARG2, FABP4 and LPL promoters in undifferentiated and differentiated ASC clones at passage 4 (P4) and at senescence (Ps)
| Percent methylated cytosines in promoter region examined | |||||||||
| Gene | LEP | PPARG2 | FABP4 | LPL | |||||
| Passage No. | P4 | Ps | P4 | Ps | P4 | Ps | P4 | Ps | |
| Undifferentiated | |||||||||
| ASC clone | B1 | 9 | 17a | 10 | 20c | 15 | 45a | 20 | 8a |
| B2 | 7 | 14a | 15 | 18ns | 30 | 25ns | 27 | 26ns | |
| B3 | 24 | 24ns | 29 | 23ns | 50 | 75a | 19 | 19ns | |
| Differentiated | |||||||||
| ASC clone | B1 | 13 | 13ns | 5 | 33a | 12 | 13ns | 17 | 11ns |
| B2 | 4 | 17a | 17 | 25c | 22 | 25ns | 29 | 24ns | |
| B3 | 22 | 38a | 17 | 22ns | 50 | 70b | 18 | 14ns | |
Chi-square tests: a P < 0.001; b P < 0.005; c P < 0.05; ns non-significant.