Figure 2.

A-ET-1 induces contraction of collagen lattices by myometrial cells. Photographs shown are of free-floating collagen lattices in culture dishes (diameter 35 mm). Collagen lattices are shown at time t = 0 (a) and after incubating for 24 h in serum-free containing 50 nM ET-1 (b). B-Effect of PRKC inhibition on ET-1-promoted gel contraction. Free-floating collagen lattices were incubated without (control) or with the cPRKC inhibitor Gö-6976 (0.5 μM), the PRKCD inhibitor Rottlerin (3 μM), the inhibitory peptide specific for PRKCZ peptide Z, the antisense oligonucleotide (AS-ODN) (4 μM) or sense (S-ODN) directed against PRKCZ before the addition of 50 nM ET-1. Results are expressed as mean percentage contraction ± SE of at least 3 determinations. *P < 0.05 compared with control.