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Coimmunoprecipitation of c-Abl and γ-PAK. c-Abl and HA-γ-PAK were transfected into 293T cells. Lysates (500 μg) were immunoprecipitated with anti-γ-PAK (N19) and control antibody (Upper) or anti-cAbl (K12) and control antibody (Lower), and the immunoprecipitates were analyzed by Western blotting as indicated; normal rabbit IgG was the control. Western blots of 10% of the original cell extracts used for immunoprecipitation (50 μg of total protein) are shown for reference.
