Figure 3.

Effects of γ-PAK on c-Abl activity and on phosphotyrosine levels. (A) c-Abl, HA-γ-PAK, and active HA-Cdc42L61 were transfected into 293T cells and analyzed by Western blotting with a mixture of PY99 and 4G10 antiphosphotyrosine antibodies. c-Abl, γ-PAK, and Cdc42L61 were detected by Western blotting with 8E9 or anti-HA antibody. (B) HA-γ-PAKwt, HA-γ-PAK K278R, and mutants of HA-Cdc42 were transfected into 293T cells. Endogenous c-Abl tyrosine kinase was assayed following immunoprecipitation of c-Abl with K12 antibodies by using GST-Crk as substrate. Lysates were simultaneously analyzed for c-Abl, γ-PAK, and Cdc42 proteins by Western blotting. Mean activation ± SEM of three independent experiments is given.