Table 1.
Primer sets for virus detection. The equivalent original volume of water analyzed for each sample ranged between 4.58 L and 6.46 L for NV, between 1.37 L and 1.94 L for HAdV and between 2.29 L and 3.23 L for HEntV.
| Virus group, primer | Sequence (5′ to 3′) | Amplicon (bp) | Reference |
|---|---|---|---|
| NV | |||
| NVp110 | AC(A/T/G)AT(C/T)TCATCATCACCATA | 398 | Le Guyader et al. 1996 |
| NVp36 | ATAAAAGTTGGCATGAACA | ||
| HAdV | |||
| AV-A1 | GCCGCAGTGGTCTTACATGCACATC | ||
| AV-A2 | CAGCACGCCGCGGATGTCAAAGT | 300 | Allard et al. 1992 |
| AV-B1a | GCCACCGAGACGTACTTCAGCCTG | ||
| AV-B2a | TTGTACGAGTACGCGGTATCCTCGCGGTC | 143 | Allard et al. 1992 |
| HEntV | |||
| ENT-up-1 | GTAGATCAGGTCGATGAGTC | Fong et al. 2005 | |
| ENT-down-1 | AC(T/C)GG(A/G)TGGCCAATC | 330 | De Leon et al. 1990 |
| ENT-up-2a | CCTCCGGCCCCTGAATG | De Leon et al. 1990 | |
| ENT-down-2a | ATTGTCACCATAAGCAGCC | 154 | Fong et al. 2005 |
a
Primers used for the second round of PCR.