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. 2007 Mar 23;367(2):419–431. doi: 10.1016/j.jmb.2006.12.070

Figure 1.

Figure 1

BcgI. The reactions contained 5 nM DNA (3H-labelled) and BcgI endonuclease (4 U) in 100 μl BcgI buffer at 37 °C. The DNA was: (a) pMLE2, a plasmid with two BcgI sites (hatch marks) and two res sites from Tn21; (b) the catenane created from pMLE2 by Tn21 resolvase, with one BcgI site in each ring. Samples (6 μl) were taken from the reactions at the times indicated, mixed immediately with 4 μl of Stop-Mix and analysed by electrophoresis through agarose. The concentrations of the following forms of the DNA were measured: (a) the intact supercoiled form of the plasmid (SC, in red), the nicked form (OC, in blue), the full-length linear form cut in both strands at one BcgI site (FLL, in green) and the mean of the two linear products cut in both strands at both BcgI sites (L1/L2, in purple); (b) the intact catenane (Cat, in red), the sum of the catenanes nicked in either one or both rings (nicked, in blue), the mean of the two linear products from double-strand breaks in the individual rings (LL/LS, in purple), the mean of the two circular products left after cutting both strands in only the opposite ring (CL/CS, in green).