Figure 2.

AloI, BaeI and BplI. Reactions contained, in 200 μl of the appropriate reaction buffer at the requisite temperature (Table 1), 5 nM ³H-labelled DNA and: (a) AloI endonuclease (1 U); (b) BaeI (1 U); (c) BplI (10 U). The DNA substrates were the SC forms of: pJM1 (blue triangles), a plasmid with one recognition site each for AloI, BaeI and BplI; pJM2 (red circles), a plasmid with two recognition sites each for AloI, BaeI and BplI (Supplementary Data, Figure S1). Samples (10 μl) were taken from the reactions at the times indicated and analysed as described in Materials and Methods. The concentrations of SC DNA still present at each time point are given as a fraction of that present at zero time.