Table 2.
Enzyme suppliers and reaction conditions
| Enzyme | Supplier | Standard reaction buffera |
|---|---|---|
| AlfI | Fermentas, Lithuania | AloI buffer with 50 μM AdoMet at 20 °C |
| AloI | Fermentas | 10 mM Tris–HCl, 100 mM KCl, 10 mM MgCl2 (pH 8.5) at 30 °C |
| BaeI | New England Biolabs, USA | 10 mM Tris–HCl, 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, 50 μM AdoMet (pH 7.9) at 25 °C |
| BcgI | New England Biolabs | 10 mM Tris–HCl, 100 mM NaCl, 10 mM MgCl2, 1 mM DTT, 20 μM AdoMet (pH 8.5) at 37 °C |
| BplI | Fermentas | 33 mM Tris–OAc, 66 mM KOAc, 10 mM Mg(OAc)2, 50 μM AdoMet (pH 7.9) at 37 °C |
| BsaXI | New England Biolabs | 20 mM Tris–OAc, 50 mM KOAc, 10 mM Mg(OAc)2, 1 mM DTT (pH 7.9) at 37 °C |
| CspCI | New England Biolabs | BaeI buffer at 37 °C |
| FalI | SibEnzyme, Russia | BcgI buffer with 50 μM AdoMet at 37 °C. |
| PpiI | Fermentas | AloI buffer at 30 °C |
| PsrI | SibEnzyme | 33 mM Tris–OAc, 66 mM KOAc, 10 mM Mg(OAc)2, 1 mM DTT (pH 7.9) at 30 °C |
a
All reaction buffers also contained 0.1 mg/ml of bovine serum albumin.