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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: J Immunol. 2006 Aug 15;177(4):2304–2313. doi: 10.4049/jimmunol.177.4.2304

FIGURE 4.

FIGURE 4

Regulation of TRAIL receptors by HIV-1 infection and TRAIL treatment. A–D, Cell surface expression of TRAIL receptor R1–4 was detected by FACS. Three days post-HIV-1 infection, control or HIV-1 infected MDM were treated with or without rhTRAIL (50 ng/ml) for 24 h. Cells were dual-immunostained for the monocyte Ag, CD14 (CD14-PE) and TRAIL receptors 1–4 (two-step staining; primary Abs, anti-TRAIL receptor 1–4 or isotype control IgG1; secondary Ab, anti-goat-FITC or anti-rabbit-FITC). Cells were gated by CD14 and fluorescent intensities of different TRAIL receptors are presented as color lines in histograms. Results are representative of four separate experiments performed with three donors.