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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: Dev Biol. 2006 Dec 15;304(1):116–126. doi: 10.1016/j.ydbio.2006.12.023

Figure 4.

Figure 4

PI3 kinase and PTEN distribution in mouse sperm. (A) Surface domains of mouse sperm. (B, C) Immunolocalization of PI3 kinase catalytic and regulatory subunits and of PTEN. For each panel >100 sperm were examined. A typical labeling pattern is illustrated in (B) and results are summarized in (C). (B) Panel shows paired phase contrast and fluorescence images (a, b; scale bar, 10 μm) and an enlarged fluorescence image of the sperm head (c; scale bar, 5 μm). Arrows facilitate orientation. Immunoblots of PI3 kinase catalytic and regulatory subunits, of PTEN, and of a control (no primary antibody). (C) Summary of immunolocalization results. Class 1A (catalytic subunits: p110α, -δ; a regulatory subunit, p85α) and IB (catalytic subunit, p110γ) PI3 kinase isoforms are detected in sperm and specifically in the acrosomal crescent region, as is PTEN. A third class IA PI3 kinase catalytic subunit (p110β) was not detected. ++, strong labeling; +, weak labeling; blank, no labeling detected.