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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: Dev Biol. 2006 Dec 15;304(1):116–126. doi: 10.1016/j.ydbio.2006.12.023

Figure 5.

Figure 5

PI3 kinase effectors, PDK1 and PKCζ, in mouse sperm. (A) PDK1 is present in the acrosomal crescent and in the flagellum. (B) PKCζ in restricted to the acrosomal crescent. (A, B) Panels show paired phase contrast and fluorescence images (scale bar, 10 μm), and a magnified fluorescence image (scale bar, 5 μm). Arrows facilitate orientation. Images are representative of 50-100 sperm observed. (C) Effects of PKC isoform-specific pseudosubstrate antagonists on the ZPse- or ZP3-evoked acrosome reaction (20 and 5 ug/ml, respectively). Myristolated (myr-) inhibitors directed against ζ, α/β, and ε isoforms were tested, as well as a myr-scrambled (scr-) PKCζ sequence and a non-myristolated PKCζ reagent. All inhibitors were used at 50 uM. Data represents the mean (± SD) of 3-5 separate experiments, with >300 sperm assessed/experiment. Dashed lines represent the levels of acrosome reaction in sperm treated with buffer or with ZPse or ZP3 in the absence of inhibitors and represent minimal and maximal responses, respectively. ZPse and ZP3 data are pooled.