Table 4.
Error frequency for classes of errors during AP site bypass
| Error frequency (%)a | ||||
|---|---|---|---|---|
| AP site | GATTGGGCb | |||
| A insertion | Deletion | ΔG (TGGC) | ∇G (TGGGGC) | |
| T7exo− DNA Pol | ||||
| GATT | ≤0.2c | ≤0.2c | 0.4 | ≤0.2 | 
| xATT | >99 | 0.6 | 0.1 | ≤0.1 | 
| GxTT | 5.5 | 85 | ≤5.1 | 1.8 | 
| GAxT | 45 | 3.5 | 45 | ≤3.4 | 
| Pol ηd | ||||
| xATC | 67 | 4.1 | nd | nd | 
| GxTC | 51 | 27 | nd | nd | 
| Dpo4d | ||||
| xATC | 69 | 22 | nd | nd | 
| GxTC | 75 | 14 | nd | nd | 
Error rates, expressed as a percentage of all events, are calculated using the formula: ((N/T) × (MF))/0.6, where N is the observed number of selected events, T the total number of sequenced samples, MF is the mutation frequency of plaque color sequenced, and 0.6 is to correct for the average minus strand expression percentage during transfection [51]. Rates are totals from sequencing of dark blue and colorless plaques.
Deletion (Δ) and addition (∇) of G at the 3G run downstream of the lesion.
Error rates at each position in the amber codon were the same.