. Author manuscript; available in PMC: 2007 Jun 15.

Published in final edited form as: DNA Repair (Amst). 2006 Jul 28;5(11):1373–1383. doi: 10.1016/j.dnarep.2006.06.003

Table 4.

Error frequency for classes of errors during AP site bypass

	Error frequency (%)^a
	AP site		GATTGGGC^b
	A insertion	Deletion	ΔG (TGGC)	∇G (TGGGGC)
T7^exo− DNA Pol
GATT	≤0.2^c	≤0.2^c	0.4	≤0.2
xATT	>99	0.6	0.1	≤0.1
GxTT	5.5	85	≤5.1	1.8
GAxT	45	3.5	45	≤3.4
Pol η^d
xATC	67	4.1	nd	nd
GxTC	51	27	nd	nd
Dpo4^d
xATC	69	22	nd	nd
GxTC	75	14	nd	nd

Error rates, expressed as a percentage of all events, are calculated using the formula: ((N/T) × (MF))/0.6, where N is the observed number of selected events, T the total number of sequenced samples, MF is the mutation frequency of plaque color sequenced, and 0.6 is to correct for the average minus strand expression percentage during transfection [51]. Rates are totals from sequencing of dark blue and colorless plaques.

Deletion (Δ) and addition (∇) of G at the 3G run downstream of the lesion.

Error rates at each position in the amber codon were the same.

Rates for human pol η [16] and S. solfataricus Dpo4 [12] shown for comparison. See footnote ‘c’ in Table 1 for comment on sequence difference.