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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: J Pharmacol Exp Ther. 2006 May 10;318(2):792–802. doi: 10.1124/jpet.106.102681

Figure 7.

Figure 7

Proteomic evaluation of rat serum by antibody array after liver injury by acetaminophen. An antibody array was used to screen for rat cytokine-chemokine expression during acetaminophen-induced liver injury from pooled rat sera from two separate studies from 6–24 hr (panel A) at n=5/group and 24 –124 hr (panel B) at n=4/group. Antibody array analysis was repeated twice for each experiment. Staining intensity by chemiluminescent detection was normalized by albumin positive controls (each blot contains 4 spots in upper left and 2 vertical spots in lower right). Open arrow (⇑) indicates MCP-1 and solid arrow (↑) indicates TIMP-1. An ELISA assay (panel C) for rat MCP-1 was used to quantitate levels from individual rats for each treatment group (mean ± SE). An asterisk (*) indicates significant difference of acetaminophen treatment (solid bar) from time-matched vehicle control (grey bar) at p<0.05 by ANOVA and post-hoc test. Panel D shows a representative antibody array blot of rat serum during acute inflammation after 2 hr and 6 hr exposure to the inflammagen, LPS (5 mg/kg, i.p.) compared to saline control (10 mg/kg, i.p.). Pooled samples (n=4/group) were used for each blot in Panel D. Cytokines were detected by chemiluminescence. Note that Panel D contains four blots; a control blot each at 2 and 6 hr and an LPS blot each at 2 and 6 hr. Cytokines are defined under Abbreviations.