Table 2.
Primary antibodies used in this study, the generating species, protein target, target amino acid sequence (if available) and species reactivities, and private vs. commercial sources.
| Primary Antibody | Type | Target | Specificity | Source |
|---|---|---|---|---|
| Ab298 | rabbit poly | Cx35/Cx36 | C-terminus aa298-318 Human (fish, rodent) | JI Nagy |
| MAB3045 | mouse mono | Cx35/Cx36 | intracellular loop; seq. NA Fish (monkey/mouse) | Chemicon |
| Ab36-4600 | rabbit poly | Cx36 | C-terminus; seq. NA Mouse | Zymed |
| Ab51-6300 | rabbit poly | Cx36 | C-terminus; seq. NA Mouse | Zymed |
| Ab37-4600 | mouse mono | Cx36 | C-terminus; seq. NA Mouse | Zymed |
| AptNR1 | rabbit poly | NR1 subunit | extraplasmic loop aa844-901, Fish | R. Dunn |
| Ab55-6308 | mouse mono | NR1 subunit | extraplasmic loop Human (rodent) | B-D Biosciences/PharMingen |
| Ab33-9100 | mouse mono | ZO-1 | aa334-6341 | Zymed |
| Ab61-7300 | rabbit poly | ZO-1 | aa463-11092 | Zymed |
| MAB3069 | mouse mono | Cx32 | cytoplasmic loop aa95-125 | Chemicon |
Monoclonal antibodies to connexins are made against target sequences of about 11–17 amino acids, whereas monoclonal antibodies to ZO-11 were made against a target sequence of 300 amino acids (Zymed Product Information Sheet), and rabbit polyclonal antibody to ZO-12 was made against a cytoplasmic sequence of > 600 amino acids (Zymed Product Information Sheet), possibly yielding additional epitope specificities, not all of which have been completely characterized (Li et al., 2004b). NA, Not available; seq., sequence; mono, monoclonal; poly, polyclonal.