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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: Biochemistry. 2006 Nov 28;45(47):14223–14231. doi: 10.1021/bi061677n

FIGURE 3.

FIGURE 3

mcfp-4 from mussel feet. (A) Acid urea-polyacrylamide gel electrophoresis of key steps in the purification of foot-derived mcfp-4. Guanidine-extracted and borate-dialyzed (pH 8.0) soluble protein stained with CBR-250 (lane 1), stained for Dopa with NBT (lane 2), and stained for histidine with Pauly’s reagent (lane 3). The remaining lanes (lanes 4-8) contained fractions under the bracketed peak of the reverse phase C8 HPLC column (B) stained with CRB-250. The double-headed arrow marks an internal calibrant, the α chain of type I collagen.