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. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: J Autoimmun. 2007 Feb 12;28(1):7–18. doi: 10.1016/j.jaut.2006.12.005

Fig. 4.

Fig. 4

Double-staining of BHK transfectant expressing mEH. After transfection, the cells were transferred to a chamber slide and cultured. Then they were fixed with acetone and double-stained by rabbit anti-mEH and mouse anti-AN mAb 1F12 (A and B), or by rabbit anti-AN antibody and 1F12 (D and E). Rabbit and mouse antibodies were detected by TRITC- and FITC-labeled second antibodies, respectively. The merged image of A and B and that of D and E are shown in C and F, respectively.