Figure 2.

XPR-1-regulated transcriptional activation of a luciferase reporter construct. Oocytes were either injected with 1 ng of XPR-1 mRNA (●) or left uninjected (□) and incubated for 16 h. Then, 3 ng of luciferase reporter construct DNA (PRE2-tata-Luc) was injected into the nucleus of each oocyte and cells were stimulated with 10 μM progesterone. At the indicated times, groups of 10 injected oocytes were collected and assayed for luciferase activity as described in Materials and Methods. Similar results were seen in two separate experiments.