Skip to main content
. Author manuscript; available in PMC: 2007 Jun 15.
Published in final edited form as: Biochim Biophys Acta. 2007 Feb 23;1772(5):580–586. doi: 10.1016/j.bbadis.2007.02.004

Figure 1.

Figure 1

Relationship of band-3 tyrosine phosphorylation, RBC deformability, and sulfate uptake. RBCs were incubated in the absence or presence of pervanadate (0-2.0 mM) and tested for protein tyrosine phosphorylation, cell deformability and sulfate uptake as described in the materials and methods section. Part A: Protein extracts from RBC ghosts were subjected to SDS polyacrylamide electrophoresis and probed with anti phospho-tyrosine antibody (Panel A upper row) or band-3 content using an anti rat band-3 antibody (Panel A lower row). Part B: RBC elongation in response to increasing shear stress in the presence of pervanadate (2.0 mM). Part C: Time dependence of sulfate uptake and the effect of DIDS (10 μM). Part D: The effect of pervanadate (2.0 mM) on RBC sulfate uptake. Mean ± SEM, from 3-6 independent assays, *significant difference compared to vehicle.